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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
X Pe Conjugated Anti Human Cd68 Kp1 Mouse Monoclonal Igg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
Anti Cd68 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of <t>CD68</t> (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of <t>CD68</t> + CD86 + and <t>CD68</t> + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.
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Image Search Results


Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of CD68 (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of CD68 + CD86 + and CD68 + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.

Journal: Open Medicine

Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats

doi: 10.1515/med-2026-1545

Figure Lengend Snippet: Macrophage-associated changes in medial collateral ligament (MCL) tissue after transplantation of ATG5-overexpressing MSCs. (A) Quantitative RT-PCR analysis of proinflammatory markers (CD80 and IL-12) and anti-inflammatory markers (CD163 and IL-10) in MCLs from aged rats on postoperative Day 14 after transplantation of MSC vector or MSC ATG5+ cells. (B) representative immunofluorescence images of CD68 (red), CD86 (green) or CD206 (green), and DAPI (blue) in MCL sections from MSC vector and MSC ATG5+ groups (scale bar: 20 μm). (C) quantification of CD68 + CD86 + and CD68 + CD206 + macrophages in MCL tissue from MSC vector and MSC ATG5+ groups. (D) ELISA analysis of pro-inflammatory cytokines (TNF-α and IL-6) and anti-inflammatory cytokines (IL-4 and TGF-β1) in MCL tissues from MSC vector and MSC ATG5+ groups. Six rats in each subgroup. were used each experiment. all quantitative data are presented as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.

Article Snippet: After blocking with 5 % BSA, the sections were incubated overnight at 4 °C with antibodies against CD68 (1:200, CST, Cat# 97778), CD86 (1:100, Thermo Scientific, Cat# RAB02398 ), and CD206 (1:400, CST, Cat# 24595).

Techniques: Tissue, Transplantation Assay, Quantitative RT-PCR, Analysis, Plasmid Preparation, Immunofluorescence, Enzyme-linked Immunosorbent Assay